Vertonghen, 33, is out of contract and has decided to move on with interest from Italy.
In his farewell message to Spurs fans, the Belgian wrote:
“So my time at the club comes to an end. A sad day for many reasons. I will miss the friends I’ve made here, the staff that make the club run, playing at the amazing new stadium & of course you fans.”
Vertonghen made 315 appearances for Spurs after joining from Ajax in 2012.